Composite

Part:BBa_K1084302:Design

Designed by: Matsumura Naoya   Group: iGEM13_HokkaidoU_Japan   (2013-09-06)


tandem RBSs for GGA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 707
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 708
    Illegal PstI site found at 722
    Illegal NotI site found at 7
    Illegal NotI site found at 715
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 708
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 708
    Illegal PstI site found at 722
    Illegal AgeI site found at 77
    Illegal AgeI site found at 134
    Illegal AgeI site found at 191
    Illegal AgeI site found at 248
    Illegal AgeI site found at 305
    Illegal AgeI site found at 362
    Illegal AgeI site found at 419
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 23
    Illegal BsaI site found at 80
    Illegal BsaI site found at 137
    Illegal BsaI site found at 194
    Illegal BsaI site found at 251
    Illegal BsaI site found at 308
    Illegal BsaI site found at 365
    Illegal BsaI.rc site found at 74
    Illegal BsaI.rc site found at 131
    Illegal BsaI.rc site found at 188
    Illegal BsaI.rc site found at 245
    Illegal BsaI.rc site found at 302
    Illegal BsaI.rc site found at 359
    Illegal BsaI.rc site found at 416


Design Notes

coming soon!


Source

coming soon!

References